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999
- 供应商:
北京泽平
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货号:BLK-180-96R-Q
中文名称:
英文名称:ROBOTIC PIPETTE TIP 300UL BLACK 96 RACK 4.8M/CS
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文献和实验High Throughput Isolation Of PCR Products Using ChargeSwitch® PCR Clean-Up
) 300 ml ChargeSwitch® Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5) 48 ml 实验设备 96 x 200 µl U-bottomed microtiter plate Any liquid
the collection rack with clean 1.5 ml screw-capped tubes into the manifold. Clamp the manifold with the white clamps, and apply 300 ul of 10:1 TE buffer heated to 65degC and pull the eluted DNA through at 5 in. Hg. After the liquid has filtered through, raise
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
over a piece of black cardboard, using a P-200 Gilson pick colony bringing with it the smallest possible volume of PBS. Pick 6 colonies in succession into a 96 well plate containing 20ul TRYPSIN/EDTA. Check using microscope that there are single colonies
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