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999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:342020-0250
中文名称:
英文名称:Media Bottle STERILE Graduated- PETG
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文献和实验Preparation of Powdered Tissue Culture Media
to 7.00 with HCl, using the pH meter. 6. Dilute the medium to the appropriate volume using a graduated cylinder and check the pH, adjusting if necessary. 7. Process the medium into sterile containers by passing through a bottle-top 0.2 um
Mixing F-12 (HAM) Media 1) Start by rinsing a graduated cylinder three times ddH2 O. 2) Then fill the cylinder to 600-ml with ddH2 O. 3) Add one bottle of F-12 (HAMS) (Sigma #N-4388). 4) Stir this until all of the powder
1 bottle L-15 (Sigma #L-4386) 4) add 7mM Hepes (1.822g for 1L) 5) pH to 7.2 with NaOH 6) bring up to 900 mls with ddH2 O-test pH 7) 450 mls media per sterile filter container 8 re-pH small sample poured into Falcon tube to assure filtering
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