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- 库存:
999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
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货号:4110-0500
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英文名称:FLASK BAFFLED PC;500ML
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文献和实验A safe method of extracting DNA from Coccidioides immitis
in the saprophytic phase at 37 C in 100 ml of 2X GYE medium (20 g glucose and 10 g yeast extract in 1 liter medium, autoclaved for 15 min.) in 500 ml plastic Nalgene flasks with plastic foam stoppers (Identi-plug, Fisher). When there has been sufficient growth
Expression Protocol in M9 Minimal Media via T7 Promoter
filter). Introduce media to a pre-autoclaved, wide-bottom (baffled) 2L flask and add ampicillin to a final concentration of 70-100 ug/ml. Grow 5mL overnight culture in same media to inoculate 500mL M9. Shake culture at 37o
Expression Protocol in M9 Minimal Media via T7 Promoter
-bottom (baffled) 2L flask and add ampicillin to a final concentration of 70-100 ug/ml. Grow 5mL overnight culture in same media to inoculate 500mL M9. Shake culture at 37oC until an OD600 of 07+/-0.2 then induce protein expression
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