赛默飞世尔Thermo Fisher THIN WALL P
IPETTE TIP 1-250ULNATURAL 96 HINGE RACK 4.8M/CS 货号：H-TW110-96RN-Q- ￥1000 - 20000
- Thermo Fisher
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- H-TW110-96RN-Q
- 2025年07月15日
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- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：H-TW110-96RN-Q
中文名称：
英文名称：THIN WALL PIPETTE TIP 1-250ULNATURAL 96 HINGE RACK 4.8M/CS
货期：热销品现货，其他请咨询
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文献和实验a chilled, sterile pipette tip to transfer the competent cells to chilled, sterile 17 x 100-mm polypropylene tubes. Store the cells on ice. Glass tubes should not be used since they lower the efficiency of transformation by ~10-fold
(250 ul/well). Pipette up and down to mix cells and PEG2. Capmat deepwells. Incubate at 30oC for 15-30 mins, on side. Heat shock at 45oC for 15 mins. Pellet cells at 3000 r.p.m. for 15 mins at least. Yeast form diffuse layer
to > 1 x 106/µg with some strains. 6. Microcentrifuge at top speed for 30 sec and remove the Transformation Mix with a micropipettor. 7. Pipette 1.0 ml of sterile water into the tube and resuspend the cells by stirring with a micropipette tip
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