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- 库存:
999
- 供应商:
北京泽平
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- 保修期:
1年
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EA
货号:TW110-96RN-Q
中文名称:
英文名称:THIN WALL PIPETTE TIP 1-250ULNATURAL 96 RACK 4.8M/CS
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文献和实验a chilled, sterile pipette tip to transfer the competent cells to chilled, sterile 17 x 100-mm polypropylene tubes. Store the cells on ice. Glass tubes should not be used since they lower the efficiency of transformation by ~10-fold
Procedures for In Vitro Production of Bovine Embryos
(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15
(250 ul/well). Pipette up and down to mix cells and PEG2. Capmat deepwells. Incubate at 30oC for 15-30 mins, on side. Heat shock at 45oC for 15 mins. Pellet cells at 3000 r.p.m. for 15 mins at least. Yeast form diffuse layer
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