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文献和实验Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
(100 units/ml and 100 μg/ml respectively) and 1.98 g of glucose to 250 ml 1x sterile phosphate buffered saline (PBS) and bring solution volume to 500 ml with additional sterile 1x PBS. 2) Prepare stop media by adding 25 ml FBS and 5 ml
Immunohistochemisty-Enzymatic Protocol
. Immunohistochem. Mol. Morphol. 8:236. Tissue Fixation and Mounting - Cryostat Sections Fix the tissue by vascular perfusion with 500 - 700 mL of Fixative. Perfuse the animal with 400 mL of Sucrose Solution. Dissect the tissue
triethylamine acetate (TEAA, Cat. No. 400613) were from Applied Biosystems and the anhydrous acetonitrile (Cat. No. 40-4050-50) was from Glen Research. Trifluoroacetic acid (TFA, Cat. No. BP618-500) was from Fisher and triethylamine (TEA, Cat. No. T-0886
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