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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:312002-0032
中文名称:
英文名称:32 oz / 1000 mL Bottle Narrow Mouth Bo
货期:热销品现货,其他请咨询




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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
Dissecting microscope; Acid Tyrode's (e.g. Sigma: T1788); Sterile plastic Petri dishes (100 x 15 mm); M2 and KSOM medium in 1 ml syringes; 9'' Pasteur pipettes; Mouth or Finger controlled pipette. Method: Place
Metabolic labeling Immunoprecipitation
/ml Cysteine-HCl,100µg/ml methionine,sterile filtered • Tris-buffered saline (TBS): 20mM Tris,150mM NaCl,pH 7.4 • X1000 Protease inhibitor soln: 500mM PMSF (Sigma P-7626)in EtOH,10mg/ml leupeptin (Sigma L-2023)in water,10mg/ml pepstatin A (P-4265
for 1/8, 1/16, and 1/32 dilutions. Repeat the entire 1/2 dilution series for the 10 dilution. You should now have twelve tubes, six for each of the 10 and the 10 dilutions. These are then diluted 1/2, 1/4, 1/8, 1/16, and 1/32. 4. Use 1.0 ml
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