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999
- 供应商:
北京泽平
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- 保修期:
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EA
货号:125AM
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英文名称:POLYSTYRENE CONTAINERS, 60ML, METAL CAP
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文献和实验Procedures for In Vitro Production of Bovine Embryos
(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15
saline (PBS) Trypsin, cold sterilized in a 125 ml sterile erlenmeyer containing a magnetic stirring bar Minimum Essential Medium Fetal Calf Serum Clinical centrifuge with sterile capped centrifuge tubes Culture flasks
for four hours in the presence of colcemid (3.125 µl of 10 µg/ml colcemid (Gibco # 15212-012) per ml of medium). 2ml per 35 mm and 4ml per 60 mm. 3) Remove media and place it in a 15 ml Falcon tube. Wash with trypsin, and trypsinize with 1.5 ml trypsin.
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