Sartorius赛多利斯VS0191VIVASPIN 50

蛋白浓缩，超滤离心管选择指南

做蛋白开发、抗体纯化，样本收集的时候，你是否经历过这些“崩溃时刻”：浓缩一管样本，膜堵了三次；好不容易浓缩完，一测浓度，目标蛋白损失大半；换缓冲液，透析过夜第二天忘了收样……样品处理看似简单，却往往是实验中最耗时、最不可控的一环。       赛米科（Cellmilk）PES超滤离心管系列，不堆砌参数，只解决实际问题：通量、回收率、操作便利度。帮你选对管子，做对实验。     1.选对截留分子量：3K、5K、10K、30K、50K、100K，孔径不是越大越好 超滤管的核心是膜孔径（即截留分子

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

complexes by pulse centrifugation (15 seconds at 3,000 RPM). Wash the bead/complexes 3 times in 500µl ice-cold TBS-mod buffer(comment 4); ;To elute the putative ChIP-Flag complexes (by competition with 3x Flag-peptide) add 100µl of TBS-mod buffer+3µl (5µg/µl

基于PCR技术的染色质沉淀分析

that was an interspecific hybrid (H) between Mus musculus domesticus (D, paternal genome) and Mus spretus (S, maternal genome). The native chromatin was then immunoprecipitated with rabbit polyclonal antisera to acetylation at lysine 9 of H3 (H3K9Ac) and to dimethylation