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- Sartorius
- VS0191
- 德国
- 2025年10月22日
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- 文献和实验
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大量
- 保修期：
两年
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- 供应商：
科邦兴业（北京）科技有限公司
- 规格：
盒
VS0191VIVASPIN 500, 3K MWCO, PES, 25/BOX.
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文献和实验complexes by pulse centrifugation (15 seconds at 3,000 RPM). Wash the bead/complexes 3 times in 500µl ice-cold TBS-mod buffer(comment 4); ;To elute the putative ChIP-Flag complexes (by competition with 3x Flag-peptide) add 100µl of TBS-mod buffer+3µl (5µg/µl
that was an interspecific hybrid (H) between Mus musculus domesticus (D, paternal genome) and Mus spretus (S, maternal genome). The native chromatin was then immunoprecipitated with rabbit polyclonal antisera to acetylation at lysine 9 of H3 (H3K9Ac) and to dimethylation
Home-made Taq Polymerase Purification
volume). This should be washed 2 x with sterile water and 4 x with ice cold Buffer B. 9) Vortex tubes well and incubate on a shaking platform (30mins / 4°C). 10) Centrifuge (approx 3K / 2min / 4°C) to pellet resin and discard supernatant. 11) Wash resin 4 x
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