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两年
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盒
93IEXS42DB-12--VSartobind® Lab S 75, strong cation exchanger, 4 mm bed height, 2.1 mL membrane volume, 75 cm²
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文献和实验capillary microcolumn is packed with two independent chromatography phases: a strong cation exchanger and reversed-phase matrix material. Once a complex peptide mixture is loaded onto the system, no additional sample handling is required
. 6. 10× Tango buffer (Thermo Scientifi c/Fermentas). 7. DTT (DL-Dithiothreitol; Cleland’s reagent). 8. 10 mM Adenosine 5′-triphosphate. 9. Ultrapure water (RNAse/DNAse-free). 10. Tris-EDTA (TE) buffer. 11. E. coli competent cells. Strains
【精华】vol 687 Chapter 4 巢式PCR侧翼序列测定实验
,in which case, proceed to the next step. 5. Use 0.5 ml of product from the preceding step to template anested 50 ml PfuTurbo?hot start PCR in (see Note 13) 1×reaction buffer including 300 mM dNTPs, 3.75 U PfuTurbo® DNA Polymerase, and 250 nM GSP2 and GSP
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