赛默飞世尔Thermo Fisher REACTI-THER

SINGLE-FLY DNA PREPS FOR PCR

for a minimum of 50 PCR analyses, and the DNA is stable for at least one month in the refrigerator. A simple modification of this technique allows the isolation of DNA suitable for use in inverse PCR (Ochman et al, Genetics 120: 621-623). These methods

SINGLE-FLY DNA PREPS FOR PCR

.   Place one anesthetized or frozen fly in each well. All rows (A-H) can be utilized, but leave the first and last columns (1 and 12) empty to ensure complete heating in step 3.   Add 50 ul of SB to each well and macerate

Preparation of Oligodeoxyribonucleotides Containing the Pyrimidine(6–4)pyrimidone Photoproduct by Using a Dinucleotide Building Block

Zhang, Y., Iwata, T., Yamamoto, J., Hitomi, K., Iwai, S., Todo, T., Getzoff, E.D., and Kandori, H. 2011. FTIR study of light‐dependent activation and DNA repair processes of (6–4) photolyase. Biochemistry 50:3591‐3598