赛默飞世尔Thermo Fisher REACTI-THER

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

by adding 1µl Rnase A (10mg/ml) and 20µl of 5M NaCl. Incubate the samples in a 65° water bath or heat block for 4-5 hours to overnight (note 5); ;After the reverse cross-linking the elutes are treated with 10µl of 0.5M EDTA, 20µl of 1M Tris-HCL, pH

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

, horizontal Gel imaging system for quantifying PCR products Heat block preset to 65°C Hemocytometer Ice Incubator, humidified, equilibrated with 5% CO2 , preset to 37°C Liquid nitrogen (optional; see Step 11) Magnetic stand

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications

of 5M NaCl. Incubate the samples in a 65° water bath or heat block for 4-5 hours to overnight (note 5);After the reverse cross-linking the elutes are treated with 10µl of 0.5M EDTA, 20µl of 1M Tris-HCL, pH 6.5 and 2µl of 10mg/ml Proteinase K