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- 文献和实验
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- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310240622
中文名称:
英文名称:15L SPINNER FLASK STANDARD 100MM NECK, WITH 2 X 45mm sidearms, 4500 complete, baffled, with a
货期:热销品现货,其他请咨询




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文献和实验Construction and Characterization of Adenovirus Vectors
. To prepare 293N3S cells in suspension: v. Transfer six 150-mm plates of confluent 293N3S cells to a 3-L spinner flask. Bring the total volume in the flask to 1 L with 1X maintenance medium.
Special Equipment Liquid nitrogen Polypropylene tubes (17 x 100 mm; Falcon 2059), chilled in ice Shaking Incubator (18ºC) Water bath preset to 42ºC
Establishment of a Primary Culture
and remove the chorioallantoic membrane, exposing the embryo. Identify and carefully remove the embryo by the neck, using a sterile metal hook or a bent glass rod, and place the embryo in a 100mm petri dish containing phosphate buffered
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