赛默飞世尔Thermo Fisher X50 96 Well
PVDF Filter Plate Polystyrene Plate with PVDF membrane white 0.2µm non-sterile 5 货号：T_70310226581- ￥16000 - 320000
- Thermo Fisher
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- T_70310226581
- 2025年07月10日
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- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310226581
中文名称：
英文名称：X50 96 Well PVDF Filter Plate Polystyrene Plate with PVDF membrane white 0.2µm non-sterile 5
货期：热销品现货，其他请咨询
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文献和实验Dual- and Triple-Color Fluorospot
can also be adjusted to single staining by using reagents for only one cytokine. 1. Dual-Color Fluorospot 1) Preparation of the plates and coating for double staining (sterile conditions) a. Pre-wet a sterile 96-well PVDF plate with 20 μl
1. Mouse L-cells (ATCC CCL-1.3 or CRL-2648). 2. Cell culture medium: Same as Section 2.1.2. 3. 96-well tissue culture plate (flat well). 4. Wnt3A samples (CM, fractions from Sections. 2.3 and 2.4 ). 5. PBS. 6. Lysis Buffer: 50 mM Tris-HCl, pH
1mg/ml collagenase type II (activity: 1.5 units/mg solid) solution, using the same cannula and another 0.2mm filter. After a further 10 mins. The ‘digest’ was then collected into a sterile 50ml centrifuge tube. More warm sterile PBS was passed
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