赛默飞世尔Thermo Fisher X4 POLYCARB

酵母转化的几种方法

storage. PEG: 400 mls 50% PEG, 50 mls 10x TE pH 7.5, 50 mls 1M LiAc pH7.5. We use relatively fresh mix, but this could be superstition. I guess you could invert, but the capmats don't seal brilliantly. Could use plate sealers (e.g. Corning Costar

【共享】关于酵母转化的几种方法，申请加分！

out quicker, so make sure incubator is humid. I had the Yale machine shop make metal holders that hold 3 deepwell boxes each. I attached these to the flat disc of a vertical rotating wheel (Glas-Col rugged rotator, from Fisher). The boxes thus get rotated

Wholemount in situ hybridisation

) 1.5µL Sp6, T7 or T3 RNA polymerase (20U/µL) 1µL   2 Incubate at 37°C for 1 hour, then add another 20U of RNA polymerase.   3 Incubate for a further hour at 37°C. 4 Remove a 1µL aliquot and run on a 1% agarose/TAE gel