赛默飞世尔Thermo Fisher 500 ML DEAE
SEPHAROSE FAST FLOW 500ML STORE AT ambient Store at Room Temperature 货号：T_70310841601- ￥1200 - 24000
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- 2025年07月11日
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- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310841601
中文名称：
英文名称：500 ML DEAE SEPHAROSE FAST FLOW 500ML STORE AT ambient Store at Room Temperature
货期：热销品现货，其他请咨询
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文献和实验in the Beckman J-6 centrifuge to pellet any cell debris. Prepare the DEAE-cellulose columns as follows: 1. Warm up the DE-52 slurry to room temperature. 2. Place the Biorad Econocolumns in the 2-row column rack and pour approximately 4 ml of the DE-52
). If such an instrumentis not available, the purification can also be performed usinga peristaltic pump that can accurately control flow rates of 1–5mL/min. The purification involves four steps: Blue Sepharose,IMAC, gel filtration, and heparin cation exchange
4.Transfer clarified extract to 50 ml tube 5.During spin prepare Ni-Sepharose: wash with 5 volumes DI water,wash with 5 volumes Binding buffer,resuspend Ni-Sepharose to 50% in Binding buffer (spins are at 500 x g for 2'-5') 6.Add 2 ml 50% Ni-Sepharose
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