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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310379650
中文名称:
英文名称:Net filter Millipore hydrophilic plain nylon 300mm x 3m roll 60µm
货期:热销品现货,其他请咨询




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文献和实验Hybridization of High Density Arrayed BAC Nylon Filter Blots
Protocol for hybridization of high density arrayed Bacterial Artificial Chromosome nylon filter blots with 100 PCR isolated Unigene cDNA inserts, pooled in a combined probe Isolation and purification of cDNA inserts using polymerase
Screening of cDNA and genomic libraries
4 to a final cons. of 10 mM Lambda plates, 1 liter. 10 g. bactotryptone 5 g. NaCl 14 g. agar. Plating out the library: Example for plating out 12 plates, 300.000
out the library: Example for plating out 12 plates, 300.000 pfu. It is important to use fresh bacterial cells. We normally plate out from 10-15 plates (140 mm) depending on the library. That is from 300,000 to 500,000 pfu. Take out 3.6 ml
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