赛默飞世尔Thermo Fisher GLOVE W/WRI

基本无菌化技术

to be put on without the bare hands ever touching the outside surface of the glove. One glove is picked up by the turned-down cuff and pul led onto the hand with the cuff left turned down (see Figure 4 - 1 and 2 ). Using the gloved hand, pick up the remaining glove

Monoclonal Antibody Production Protocol

(1)Cell Fusion and Selection Solutions: - IMDM - IMDM complete + 15% MCM. - PBS - PEG 50% ( w/v ): PEG 4000 ( 50% ) from Boehringer Manheim 1 243 268 - Aminopterin: Sigma, A 5159 (50X ) Prepare a T-150 flask with 170ml of IMDM

小RNA的Northern BLOT

#______________  1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________  X μl Nuclease free H2O to 10 μl  37℃ at least 1 hr  After incubation, bring reaction volume up to 50 μl before cleaning up with spin column