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999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310034560
中文名称:
英文名称:X50 Filters polycarbonate Isopore membrane plain white 142mm dia5.0µm pore size (pack of 50)
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文献和实验by centrifuging the gradient at 100,000 x g (28,000 rpm)for 3 h at 4℃ in a SW-28 rotor. 5.In the cold room,with a Pasteur pipette carefully harvest the off-white band of Golgi membrane at the 0.25 M/1.1M sucrose interface and the off-white band of ER (ER)membrane
both membrane fractions with 3 volumes of 0.25 M sucrose in homogenization buffer containing 0.5 mM GTP,and pellet the membranes by centrifugation at 100,000 x g (29,000 rpm)for 1 h at 4℃ in a 50.2Ti rotor. 8.In the cold room,discard the supernatants
Preparation of Rat Liver Cell Cytosol
at 100,000 x g (28,000 rpm) for 3 h at 4oC in a SW-28 rotor. In the cold room, with a Pasteur pipette carefully harvest the off-white band of Golgi membrane at the 0.25 M/1.1M sucrose interface and the off-white band of ER (ER) membrane
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