Sartorius赛多利斯SDLV-0500-20E0-ES

碱裂解法大量提取质粒（500mL菌液）

的无水乙醇，-20℃静置20min使质粒沉淀。 10、同7步，并将质粒溶液中加入20μL的RNaseA(终浓度20μg/mL)，37℃放置0.5-1h。 11、分别加入等体积萃取液(酚：氯仿：异戊醇=25：24：1)，振荡混匀，配平，4℃下8500rpm离心10 min。 12、取上层水相至新的EP管中，加入等体积氯仿/异戊醇(24：1)，振荡混匀，配平，4℃下8500rpm离心10 min。 13、取上层水相于新的EP管中。注意：13

Partial proteolysis with Staph. aureus V8 protease

. If you have too much, the V8 won't work. The molarity of neat mercaptoethanol is approximately 14. 1 microliter of ME per 14 ml of solution gives a 1 mmolar solution.   Enzyme stock solution: 500 micrograms/ml (or 500 nanograms/microliter) V8-protease in 125

Northern Blotting (Howell Lab)

before quantitation. Pipet up and down several times before taking 2 µl into 998 µl water. Read at 260 nm. RNA concentration (µg/µl) = OD x 500 x 0.04 µg/µl = OD x 20 Formaldehyde gel Boil 2 g agarose in 144 ml water (check weight