Sartorius赛多利斯93IEXD42DB-12--VS
artobind® Lab D 75, weak anion exchanger, 4 mm bed height, 2.1 mL, 75 cm²- ￥2769
- Sartorius
- 93IEXD42DB-12--V
- 德国
- 2025年10月21日
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大量
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两年
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93IEXD42DB-12--VSartobind® Lab D 75, weak anion exchanger, 4 mm bed height, 2.1 mL, 75 cm²
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文献和实验Wholemount in situ hybridisation
the embyros at 4°C or -20°C at this point, for up to a few months, or preferably in pre-hyb (see step 12). Day 1 5 Rehydrate by taking the embryos back through a MeOH/PBTX (75%MeOH -> 50% MeOH -> 25% MeOH -> PBTX) series in reverse
Large Scale Tubulin Preparation
. Frozen brains do not work for preparing tubulin. The best preps have been done with freshly removed brains transported in an ice-filled cooler to the lab within 1-2 hours of removal. For transporting 10-12 brains from the slaughterhouse, we use 2 size 16
Large Scale Tubulin Preparation
: 151.2 grams PIPES, free acid 3.8 grams EGTA 2 ml of 1 M MgCl2 pH with KOH to pH 6.75, and bring up to 1 liter. Check pH at 1X is 6.7 Make 3.5 liters of 10X CB for 10-12 brain prep. GTP : Sigma Type IIS- # G-8752 ATP : Sigma
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