赛默飞世尔Thermo Fisher HPLC column

色谱柱和色谱系统的故障检修HPLC Column and System Troubleshooting

Injector Seal Failure进样器密封性差 Column: Bonus-RP, 4.6 × 75mm , 3.5µm Mobile Phase: 30% H2O : 70% MeOH       Flow Rate: 1.0 mL/min         Temperature: R.T.                                    Detection: UV 254 nm   Sample: 1. Uracil  尿嘧啶                    

A High‐Throughput Screening Method for Identification of Inhibitors of the Deubiquitinating Enzyme USP14

(2 µg), and 1 µg of purified Ub‐AMC and HA‐tagged Ub‐AMC (HA‐Ub‐AMC). Free Ub (1 µg) was loaded for comparison. (C ) SDS‐PAGE analysis and CBB staining of purified USP14 WT and CA. A known amount of BSA was loaded for comparison. RP2 CP indicates doubly

Convenient and Efficient Approach to the Permanent or Reversible Conjugation of RNA and DNA Sequences with Functional Groups

for 6 hr at 55°C. (C ) Chromatogram of a commercial sample of uridine. Conditions: RP‐HPLC analysis is performed using UV detection (254 nm) and a 5 µm Supelcosil LC‐18S column (25 cm × 4.6 mm) according to the following method: starting from 0.1 M