赛默飞世尔Thermo Fisher X96 PIPETTE
TIP ZIPTIP WITH 0.6µL STRONG CATION resin device material: polypropylene non-sterile 货号：T_70310748354- ￥1200 - 24000
- Thermo Fisher
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- T_70310748354
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310748354
中文名称：
英文名称：X96 PIPETTE TIP ZIPTIP WITH 0.6µL STRONG CATION resin device material: polypropylene non-sterile
货期：热销品现货，其他请咨询
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文献和实验Method for Single-Cell Electroporation
vectors containing promoters appropriate for tissue type. We purify our plasmid DNA using Promega Wizard Plus MidiPreps DNA purification system (Promega, Madison, WI). We dilute purified DNA to 0.2-1 µg/µl and fill the micropipette tip with 0.6-1 µl
capacity and a loading volume of up to 800 µl. Fused-silica capillary, 100 µm I.D. x 365 µm O.D. (Agilent Technologies or Polymicro Technologies) Fused-silica capillary (size and length depend on the desired split flow rate, see Step 10) Gold
and add 2 L of 0.1 M MgSO4. Mix, settle, aspirate, and vacuum filter as in step 1. Transfer the phosphocellulose resin to the 4 L beaker, add 2 L of 10 x column buffer. Mix gently for 10-15 min, allow the resin to settle, aspirate excess column
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