赛默飞世尔Thermo Fisher X8 Pipette
tip ZipTip with 0.6µL strong cation resin device material: polypropylene non-sterile 货号：T_70310453382- ￥70 - 1400
- Thermo Fisher
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- T_70310453382
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310453382
中文名称：
英文名称：X8 Pipette tip ZipTip with 0.6µL strong cation resin device material: polypropylene non-sterile
货期：热销品现货，其他请咨询
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文献和实验Method for Single-Cell Electroporation
cultures. Targeting transcription to individual cells is achieved by restricting both the DNA and the electric field required for electroporation to the 0.6-1 µm tip of a glass micropipette. This technique is relatively easy to setup and perform, can yield
capacity and a loading volume of up to 800 µl. Fused-silica capillary, 100 µm I.D. x 365 µm O.D. (Agilent Technologies or Polymicro Technologies) Fused-silica capillary (size and length depend on the desired split flow rate, see Step 10) Gold
and add 2 L of 0.1 M MgSO4. Mix, settle, aspirate, and vacuum filter as in step 1. Transfer the phosphocellulose resin to the 4 L beaker, add 2 L of 10 x column buffer. Mix gently for 10-15 min, allow the resin to settle, aspirate excess column
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