赛默飞世尔Thermo Fisher X2 Disposab

原创：western protocol

Tris(PH=6.8) 10% SDS 40 µl 10% ammonium persulfate 40 µl TEMED 7 µl C. Preparation of gel 1.  Assemble the glass plates and spacers (0.75 mm thick). 2.  Pour the running gel to about 1 cm below the wells of the comb (~3.2 ml). 3.  Seal with 1 ml

Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shea

. 4).     10)  Place a sterile syringe filter into the air vent (Fig. 4).     11)  Move the flow circuit into a humidified incubator at 37 °C, 5% CO2 .     12)  Clamp the hard tubing into the roller pump head indicated for use with this type

Procedures for In Vitro Production of Bovine Embryos

. Prepare 1 M stock by adding 0.735 g CaCl2 +2H2 O to 5 ml water. Sterile filter and store at 4°C indefinitely.Stock 14: Hyaluronidase. Prepare stock solution of type IV hyaluronidase at 10,000 units/ml in saline, sterilize through a 0.2 mm