赛默飞世尔Thermo Fisher X20 SPE col

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in water gradient, both containing 0.1% (v/v) TFA, at 1 mL/min over 30 minutes at 60 °C with a C18, 5 μm, 50 X 2 mm column) and MALDI (Fig. 5). Purification with reverse phase HPLC on a Vydac C18 column (10 μm, 22 mm x 250 mm) proceeded, using

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Parafilm 65°C water bath Spectrophotometer (Thermo Scientific, cat. no. 840‐208200

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Porous graphitized carbon (PGC) analytical HPLC column (Thermo Scientific) High‐performance liquid chromatography (HPLC