赛默飞世尔Thermo Fisher HEATING BLO
CK CORNING SINGLE FOR 12 X 17MM TUBES for Corning LSE dry bath heater 货号：T_70311363825- ￥1200 - 24000
- Thermo Fisher
- 美国/芬兰/中国/韩国/墨西哥
- T_70311363825
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70311363825
中文名称：
英文名称：HEATING BLOCK CORNING SINGLE FOR 12 X 17MM TUBES for Corning LSE dry bath heater
货期：热销品现货，其他请咨询
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文献和实验4 ul of fly supernatant to 16 ul of 1.25X NdeII buffer (125 mM Tris-HCl pH 7.6, 12.5 mM MgCl[2], 188 mM NaCl, 1.25 mM DTT). Add 0.5 ul of NdeII (BRL) and incubate at room temp. for 15 min. Inactivate the enzyme by heating to 65 C for 15 min.
Add 4 ul of fly supernatant to 16 ul of 1.25X NdeII buffer (125 mM Tris-HCl pH 7.6, 12.5 mM MgCl[2], 188 mM NaCl, 1.25 mM DTT). Add 0.5 ul of NdeII (BRL) and incubate at room temp. for 15 min. Inactivate the enzyme by heating to 65 C for 15 min
Single-molecule analysis of DNA replication by molecular combing
. Use a Pasteur pipette rubber bulb to eject plugs directly into 12 ml round-bottom tubes containing Zymolyase buffer (0.4 ml per plug). Incubate overnight at 37°C. Replace Zymolyase buffer with Proteinase K buffer (0.4 ml per plug
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