赛默飞世尔Thermo Fisher X1000 Heat
shrink band Nalgene for Nalgene PETG media bottles fits closure size 20-415 (pack of 货号：T_70310424553- ￥1000 - 20000
- Thermo Fisher
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- 2025年07月12日
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- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310424553
中文名称：
英文名称：X1000 Heat shrink band Nalgene for Nalgene PETG media bottles fits closure size 20-415 (pack of
货期：热销品现货，其他请咨询
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文献和实验Large Scale Plasmid Preps: Qiagen/Cesium Method
transfections of mammalian cells. PROCEDURE: Grow 250 - 500 mL of bacteria overnight in LB with 50 µg/mL of ampicillin. Transfer culture to Nalgene bottles. Spin in SGA rotor at 6000 rpm, at 4ºC for 10 min. Purify the plasmid
Marcantonio Lab Protocol Manual
for 15 minutes in Sepcor bottles. 3) Carefully pour off supernatant and aspirate off any media remaining. 4) Resuspend pellet in 4 ml of ice-cold GTE by pipetting up and down. Let stand 5 minutes at room temperature. Be sure to completely resuspend
sodium acetate solution to pH 6.0 with the 3M acetic acid solution. Filter sterilize using a 0.2 micron filter. Store at room temperature. Ethanol/acetate mix Ethanol (100%) 950 ml Sodium acetate pH=6.0 50 ml 1000 ml 3X SSC
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