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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310640493
中文名称:
英文名称:X50 TLC plates Macherey-Nagel silica gel 60A 5cm x 20cm TLC plates flexible polyester sheets with
货期:热销品现货,其他请咨询




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文献和实验Carbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC Plates
on ice 150mm Petri dishes Glutaraldehyde X-ray film (or alternate cell detection system) Protocol: 1. Prescored 10 x 10 cm HPTLC plates are broken along one score to generate a 5 cm x 10 cm
in 30 µl Ethyl Acetate. 19. Spot on silica gel TLC (in 5 µl amounts). 20. Develop TLC in 200 ml Chloroform/Methyl-OH (95:5) until solution is almost at the top (app. 1 hr.). 21. Dry & expose against film ON.
. Vortex 10" each tube & then mix by inverting for another 30". 15. Spin @ 14K RPM for 30"-1'. 16. Remove 450 µl of organic (upper) phase. 17. Speed vac for 15'. 18. Resuspend in 30 µl Ethyl Acetate. 19. Spot on silica gel TLC (in 5 µl amounts
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