赛默飞世尔Thermo Fisher X960 Pipett
e tips MBP racked 1-300µL low retention sterile softfit (pack of 960) 货号：T_70310110062- ￥70 - 1400
- Thermo Fisher
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- T_70310110062
- 2025年07月14日
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- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310110062
中文名称：
英文名称：X960 Pipette tips MBP racked 1-300µL low retention sterile softfit (pack of 960)
货期：热销品现货，其他请咨询
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
SDS lysis buffer/1x proteinase inhibitor cocktail (2.5 µl of 200 x stock from Upstate EZ ChIP kit). Roughly dissociate heads by pipetting, and transfer them into a 2 ml dounce homogenizer. Total volume is ~700 µl. �SDS lysis
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge
Transformation of Electrocompetent E. coli with Blue/White selection
Desalt DNA template by EtOH precipitation in NaOAc followed by at least 2x washes with 70% EtOH. Resuspend in 5 - 15 µL of sterile H2 O. Rinse cuvettes (if they have been used before) 5x with deionied H2 O, and place them on ice
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