赛默飞世尔Thermo Fisher VIAL 16ML 2

Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shea

medium (MCDB-131 medium with 5 ml of 200 mM L-glutamine, 10% FBS and EGM-2 SingleQuots) at 37 °C, 5% CO2 .     5)      Slowly change medium every 24 hours for the first 7 days, then every other day. EPC colonies can be identified after an average

单细胞基因表达分析

of the extractant must be optimized for each cell type to obtain optimal reduction of cytoplasmic background without damaging nuclei or loss of cells. 6. Fixative (4% PFA): To make 50 ml, take one 10 ml vial of 20% paraformaldehyde stock, add 5 ml 10X PBS stock

Single Cell Gene Expression Profiling: Multiplexed Expression Fluorescence in situ Hybridization (FISH)

. 4. Filtration column running buffer (10mM TEAB): To make 1 liter, take 5 ml of 2M TEAB stock solution and complete to 1 liter with DDW. Store at 4°C. 5. Filtration column running matrix: To make approximately 200 ml, pour 200