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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70314895561
中文名称:
英文名称:X190 Specimen Tubes 14ml Neutral Glass Squat Form without closure (Pack of 190)
货期:热销品现货,其他请咨询




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文献和实验电镜样品制备方法 Specimen preparation for electron microscopy
into approx. 1mm cubes for fixing. This may be done in a drop of fix on a sheet of dental wax, using a razor blade. Place in glass processing vials and close with plastic caps. (Tissue may be stored at this stage.) Wash in 0.1M buffer - 1 hour x
RNA purification using TRizol--TRIZOL法提取RNA
microcentrifuge tubes.b.Centrifuge for 1 minute to pellet the cells.c.Pour off the supernatant.d.Add 1 ml of TRIzol or TRI reagent to the tubes.e.Lyse cells by repetitive pipetting.f.Centrifuge homogenate at 12000 x g for 10 minutes at 4℃.g.Transfer the homogenate
on a sheet of dental wax, using a razor blade. Place in glass processing vials and close with plastic caps. (Tissue may be stored at this stage.) Wash in 0.1M buffer - 1 hour x 2. (Or overnight at 4o C.) Post fix in osmium tetroxide in 0.2
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