赛默飞世尔Thermo Fisher X960 Pipett

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

(9)  Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make

DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)

of the reagent per 2 x 106 of cultured cells.    2) Cells Grown in Monolayer Lyse cells directly in a culture dish by adding 300 μL of Cell Lysis Buffer per 2 x 106 of cultured cells directly into each well of multiwell cell culture plate or flask

Method for Single-Cell Electroporation

(here, either intact tadpole, or rat hippocampal slice culture) is placed under a dissecting microscope or an upright Olympus BX50 microscope equipped with a 20X long working distance objective. Using visual guidance at low magnification, the tip of the DNA