Thermo Fisher 5-60 ml 瓶口分液器吸入延

人单核细胞和中性粒细胞

(1000X) 0.5 ml + Glutamine (100X) 5 ml + 5% FBS (25 ml) ProcedureDraw venous blood into 60 ml syringe with 10ml 6% dextran + 7 ml citrate/citric acid. Fill to 60 ml total (blood 43 ml).Mix and sediment 30 min at RT (white blood cells now with serum

A quick RNA mini-prep for Neurospora mycelial cultures

. Mortar and pestle should be washed after every sample. 4. Working quickly before powder can thaw, pour or spoon ground mycelia into 15 ml round bottom Sarstedt tube (Sarstedt tubes #60.540, Princeton, NJ) containing 8 ml of E buffer at room

Pheromone Halo Assay

into a 5 or 14 ml plastic tube with a pop-off cap (e.g. Falcon, #35-2063). 5. Immediately before performing the assay, transfer a small volume of the saturated starter culture into the tube containing agar. 100 ul of starter culture should be used