赛默飞Ezy-Cal计数控制微粒，25µm，2000/ml，

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2

RNA In Situ Hybridization of Dissected Gonads with Digoxygenin-Labeled Probes

Sciences #16220)   25 ml 0.2 M K2HPO4 (pH7.2)   15 ml ddH2 O   Dispense in 10 ml aliquots and store at -20 C.       Proteinase K ; 20 mg/ml. Aliquoted in 10 ul and stored at -80 o C

Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos

7.0 µl 10x Taq Buffer 2.5 µl 25 mM MgCl2 1.5 µl 10 x dNTP mix 5.0 µl Primer * (30 ng/µl) 5.0 µl Digested DNA (100-200 µg/ml) 2.0 µl mineral oil 40 µl * For cDNAs cloned in Bluscript, we use the following primers (21mers): "T