相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
CDK2/CyclinE1, Active
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
10 µg
蛋白名称:CDK2/CyclinE1, Active
蛋白构建:Recombinant full length human CDK2 and CyclinE1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.
表达宿主:Baculovirus-Insect Cells
蛋白分子量:CDK2 ~58 kDa; CyclinE1 ~73kDa
蛋白NP号:NM_001798 ,NM_001238
蛋白氨基酸序列:Full Length
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Assays for Cyclin-Dependent Kinase Inhibitors
several substrates including Rb. On the other hand, CDK2/cyclin A complex is active throughout S phase. For cells to progress to mitosis, CDK1 (CDC2) must be “activated,” and activated cdc2 phosphorylates substrates required for mitosis (3 ).
Analysis of Cyclin-Dependent Kinase Activity
of the cell cycle that require their kinase abilities—CDKs 3, 4, and 6 are active during G1 phase, CDK2 functions during the G1-to-S transition and S phase, and cdc2 (also known as cdk1) is essential for the G2-to-mitosis transition ( 3 ). In vivo
X-100或脱氧胆酸钠)和低浓度变性剂(2mol/L尿素或盐酸胍等)洗涤除去脂类和膜蛋白,这一步很重要,否则会导致包涵体溶解和复性的过程中重组蛋白质的降解[6、7、8]。 包涵体的溶解必须用很强的变性剂,如8mol/L尿素、6~8mol/L盐酸胍,通过离子间的相互作用破坏包涵体蛋白间的氢键而增溶蛋白。其中尿素的增溶效果稍差,异氰盐酸胍最强;去污剂,如SDS[7],可以破坏蛋白内的疏水键,可以增溶几乎所有的蛋白,但由于无法彻底去除而不允许用在制药行业中;酸,如70%甲酸[9],可以破坏
技术资料暂无技术资料 索取技术资料











