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- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
CDK2/CyclinE2, Active
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
10 µg
蛋白名称:CDK2/CyclinE2, Active
蛋白构建:Recombinant full length human CDK2 and CyclinE2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.
表达宿主:Baculovirus-Insect Cells
蛋白分子量:CDK2 ~58 kDa; CyclinE2 ~72 kDa
蛋白NP号:NM_001798 ,NM_057749
蛋白氨基酸序列:Full Length
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文献和实验Assays for Cyclin-Dependent Kinase Inhibitors
of the Retinoblastoma gene product (Rb) by CDK4 and/or CDK6 (G1/S checkpoint), leading to the release of bound E2F from Rb and allowing the transcription of genes by “free E2F” necessary for S phase progression. During late G1 phase, CDK2/cyclin E complex phosphorylates
3 篇 Nature 连发,解决世纪难题,周期蛋白的毁灭调控蕴含癌症患者的新生
在 cyclin D 上,被泛素化标记的 cyclin D 将会被蛋白酶体降解。此外研究还发现,AMBRA1 缺失会导致 cyclin D 和 MYC 蛋白水平升高。cyclin D 与 CDK4/6 结合,使 RB1 蛋白磷酸化。磷酸化 RB1 释放 E2F 转录因子来驱动细胞周期进程所需基因的表达。在 AMBRA1 缺失的细胞中,cyclin D 也能与 CDK2 激酶形成复合物,使癌细胞能够抵抗 CDK4/6 抑制剂的治疗。高水平的 cyclin D 会促进细胞增殖,从而导致 DNA 损伤、复制应激
Analysis of Cyclin-Dependent Kinase Activity
of the cell cycle that require their kinase abilities—CDKs 3, 4, and 6 are active during G1 phase, CDK2 functions during the G1-to-S transition and S phase, and cdc2 (also known as cdk1) is essential for the G2-to-mitosis transition ( 3 ). In vivo
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