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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Stampwell-larvae 2
- 保质期：
长期
- 供应商：
北京博蕾德生物科技有限公司
- 保存条件：
常温
- 规格：
1 Unit Pack
凝胶孔制备,用于斑马鱼幼虫和胚胎成像和定位。
Stampwell stamps imprint arrays of wells in hydrogels in just one time. Nothing more to do than load your samples into the wells and image. The Stampwell shapes have been optimized for the imaging of fish embryos or larvae up to 20 dpf.
Save time: no need to manually position your samples individually. Fish embryos/larvae will self-position in the wells.
Boost your assay reproducibility: once loaded in the wells, your samples are perfectly positioned, aligned and located on a similar focal plane.
Open system: direct access to embryos/larvae for adding external compounds (i.e. drug screening).
4 different shapes
depending on the size and orientation of your fish embryos.
Embryo 1 (ie Rectangular)
. Number of pins: 35
. Shape of the pins: rectangular
. Length * width of the wells: 2 mm * 0,65 mm
Embryo 2:
. Number of pins: 18
. Shape of the pins: a drop
. Length of the wells: 3.90 mm
. Largest width of the wells: 0.88 mm
Larvae 1:
. Number of pins: 5 large pins ideal for the 9-14 dpf zebrafishes + 5 medium pins ideal for the 6-9 dpf zebrafishes + 5 small pins ideal for the 3-6 dpf zebrafishes
. Shape of the pins: fish body and tail
. Length of the 5 large wells: 6 mm, 2.7 for the body and 3.3 for the tail
. Length of the 5 medium wells: 5 mm, 2.25 for the body and 2.75 for the tail
. Length of the 5 small wells: 4 mm, 1.8 for the body and 2.2 for the tail
Larvae 2:
. Number of pins: 5 large wells ideal for the 15-20 dpf zebrafish or 39-42 dpf medaka larvae + 5 small wells ideal for the 1-2 dpf zebrafish
. Shape of the pins: prism
. Length of the 5 large wells: 10 mm
. Length of the 5 small wells: 3 mm
. Well depth: 1 mm
Plate compatibility:
The stamp has a total diameter of 26 mm. It can be used in:
- 35 mm circular petri dishes with an aperture of at least 22 mm
- any larger vessel (i.e. 6-well plates)
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文献和实验Stampwell has been originally developed by Gaelle Recher and published in Scientific Reports:
Alessandri K, Andrique L, Feyeux M, Bikfalvi A, Nassoy P, Recher G. All-in-one 3D printed microscopy chamber for multidimensional imaging, the UniverSlide. Sci Rep. 2017 Feb 10;7:42378. PMID: 28186188; PMCID: PMC5301227.
Publications from Stampwell users:
Asad A, Shahidan NO, de la Vega de Leon A, Wiggin GR, Whitfield TT, Baxendale S. A screen of pharmacologically active compounds to identify modulators of the Adgrg6/Gpr126 signalling pathway in zebrafish embryos. Basic Clin Pharmacol Toxicol. 2023;133(4):364‐377.
Sahai-Hernandez P, Pouget C, Eyal S, Svoboda O, Chacon J, Grimm L, Gjøen T, Traver D. Dermomyotome-derived endothelial cells migrate to the dorsal aorta to support hematopoietic stem cell emergence. Elife. 2023 Sep 11;12:e58300.PMID: 37695317; PMCID: PMC10495111.
Merdrignac C, Clément AE, Montfort J, Murat F, Bobe J. auts2 Features and Expression Are Highly Conserved during Evolution Despite Different Evolutionary Fates Following Whole Genome Duplication. Cells. 2022 Aug 30;11(17):2694. PMID: 36078102; PMCID: PMC9454499.
Baculovirus Insecticide Production in Insect Larvae
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Focal Electroporation in Zebrafish Embryos and Larvae
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Cuticle Preparation of Drosophila Embryos and Larvae
. Here, I describe methods of preparation and analysis of cuticles from 1st instar larvae as well as from 3rd instar larvae. In addition, a protocol to simultaneously analyze cuticles and β-galactosidase activity of embryos expressing lacZ reporter genes is presented.
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