- ￥1280 - 2180
- Tanda
- 中国
- TD-31803
- 2025年11月26日
- FCM、ELISA、IHC、WB
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
peptide
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
-20℃
- 克隆性：
mAb
- 适应物种：
Human
- 保质期：
1年
- 目录编号：
TD-31803
- 级别：
科研级
- 库存：
88
- 供应商：
武汉天德
- 宿主：
Mouse
- 应用范围：
FCM、ELISA、IHC、WB
- 靶点：
详询
- 抗体英文名：
Mouse Monoclonal Antibody to CD163
- 抗体名：
CD163
- 规格：
100ul/50ul
| 规格： | 100ul | 产品价格： | ￥2180.0 |
|---|---|---|---|
| 规格： | 50ul | 产品价格： | ￥1280.0 |
Mouse Monoclonal Antibody to CD163
Description |
|
| The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. | |
Specification |
|
| Aliases | M130; MM130; SCARI1 |
| Entrez GeneID | 9332 |
| Swissprot | Q86VB7 |
| clone | 7G5E2 |
| WB Predicted band size | 125.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Purified recombinant fragment of human CD163 (AA: extra 42-259) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
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文献和实验Monoclonal Antibody Production
Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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