- ￥1280 - 2180
- Tanda
- 中国
- TD-30240
- 2026年04月11日
- FCM、ELISA、IHC
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
peptide
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
-20℃
- 克隆性：
mAb
- 适应物种：
Human
- 保质期：
1年
- 目录编号：
TD-30240
- 级别：
科研级
- 库存：
88
- 供应商：
武汉天德
- 宿主：
Mouse
- 应用范围：
FCM、ELISA、IHC
- 靶点：
详询
- 抗体英文名：
Mouse Monoclonal Antibody to CD133
- 抗体名：
CD133
- 规格：
100ul/50ul
| 规格： | 100ul | 产品价格： | ￥2180.0 |
|---|---|---|---|
| 规格： | 50ul | 产品价格： | ￥1280.0 |
Mouse Monoclonal Antibody to CD133
Description |
|
| This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. | |
Specification |
|
| Aliases | RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061 |
| Entrez GeneID | 8842 |
| Swissprot | O43490 |
| clone | 3F10 |
| WB Predicted band size | 133kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide of human CD133. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
Application |
|
| IHC | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
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