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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
peptide
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
-20℃
- 克隆性:
mAb
- 适应物种:
Human
- 保质期:
1年
- 目录编号:
TD-R26532
- 级别:
科研级
- 库存:
88
- 供应商:
武汉天德
- 宿主:
Rabbit
- 应用范围:
WB,IHC-P,IP
- 靶点:
详询
- 抗体英文名:
CD105
- 抗体名:
CD105
- 规格:
100ul/50ul/20ul
| 规格: | 100ul | 产品价格: | ¥2280.0 |
|---|---|---|---|
| 规格: | 50ul | 产品价格: | ¥1280.0 |
| 规格: | 20ul | 产品价格: | ¥658.0 |
CD105 Rabbit mAb
|
Product Name
|
CD105 Rabbit mAb
|
|
Gene ID
|
2022
|
|
SwissProt ID
|
P17813
|
|
Gene Name
|
ENG
|
|
Alternative Names
|
ENG; END; Endoglin; CD antigen CD105
|
|
Background
|
Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
|
|
Research Field
|
Epigenetics and Nuclear Signaling
|
|
Product Categories
|
Primary antibody
|
|
Host
|
Rabbit
|
|
Reactivity
|
Human
|
|
Application
|
WB,IHC-P,IP
|
|
Dilution Ratio
|
WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20
|
|
Molecular Weight
|
Calculated MW: 71 kDa; Obs/erved MW: 95 kDa
|
|
Clonality
|
Monoclonal Antibody
|
|
Clonality No.
|
R05-3H7
|
|
Isotype
|
IgG
|
|
Immunogen
|
Recombinant protein of human CD105
|
|
Purification
|
Affinity Purified
|
|
Conjugation
|
Unconjugated
|
|
Modification
|
Unmodified
|
|
Form
|
Liquid
|
|
Buffer System
|
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
|
|
Storage
|
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
|
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文献和实验Purification of Adipose Tissue Mesenchymal Stem Cells and Differentiation Toward Hepatic-Like Cells
yet; therefore, it is still not obvious how to purify these heterogeneous fraction of cells. We postulate that one of the markers defining MSC provenance is CD105 (endoglin). Therefore, we have sorted CD105+ fraction of AT-MSCs, expanded them, and differentiated
Derivation and Characterization of Human ESC-Derived Mesenchymal Stem Cells
. Repeated passaging by trypsinization results in a highly enriched MSC culture. Enriched MSC cultures can be further purified to homogeneity by limiting dilution or FACS sorting for a CD105+ or CD73+ and CD24− cell population. The resulting hES-MSCs fulfill
骨活性。 从上图A可以看出流式分选仪以CD105作为表面标志分选出阳性hADSCs细胞,其比例约占单核细胞50%;B流式细胞仪验证磁珠分选CD105*hADSCs细胞纯度可达80%;C茜素红染色示成骨诱导后CD105*hADSCs组钙结节表达;D茜素红染色示成骨诱导后未分选hADSCs组钙结节表达。流式分选样本常规处理方法: 1、分选外周血,骨髓时:加抗凝剂,去红细胞 2、分选组织时,机械分离或胶原酶 3、培养细胞,使用胰酶消化 在实际细胞分离中给,因为流式
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