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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
- 英文名:
Win 24540 (Standard)
- 库存:
货期:询盘
- 供应商:
MedChemExpress LLC
- CAS号:
13647-35-3
- 规格:
5 mg/10 mg/25 mg/50 mg/100 mg
| 规格: | 5 mg | 产品价格: | ¥500.0 |
|---|---|---|---|
| 规格: | 10 mg | 产品价格: | ¥750.0 |
| 规格: | 25 mg | 产品价格: | ¥1350.0 |
| 规格: | 50 mg | 产品价格: | ¥1890.0 |
| 规格: | 100 mg | 产品价格: | ¥2400.0 |
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文献和实验Standard Protocols Autoradiography (35S)
Remove Kodak NTB2 nuclear emulsion from fridge and place at 42o C for around 30-60 mins (until melted). Make up the developer and the fixer and place in the darkroom ready for later (only if developing the same day
- 72oC for 30 - 60 sec (anneal) 72oC for 30 - 60 sec (elongate) (60 sec per kb target sequence length) 25 - 35 cycles only (otherwise enzyme decay causes artifacts) 72oC for 5 min at end to allow complete elongation of all product DNANOTE
Thermal Cycling Profile for Standard PCR
that is easily detectable on a gel stained with ethidium bromide. Most PCRs should, therefore, include only 25 to 35 cycles. As cycle number increases, nonspecific products can accumulate (see figure below).
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