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文献和实验" wax gem and remove; do as for oil or bottom-puncture tube and blow out aqueous drop for Vaseline. Cleaning-up DNA: 3 options A protocol which gives DNA that is clean enough for sequencing is the following: increase volume to 100ul with water, add
Steps for Standard PCR Reaction Design primers. In general, primers should have the following properties: Length of 18-24 bases 40-60% G/C content Start and end with 1-2 G/C pairs
Standard Protocols Autoradiography (35S)
). The developer should be a 1:1 dilution in water. The fixer is 30% (w/v) of sodium thisulphate. Place slides in a dark box and carry to dark room. Also take a slide rack, tweezers and the emulsion. Switch on the red light and switch
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