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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
- 英文名:
DU-176 (Standard)
- 库存:
货期:1-2天
- 供应商:
MedChemExpress LLC
- CAS号:
480449-70-5
- 规格:
5 mg/10 mg/25 mg/50 mg/100 mg
| 规格: | 5 mg | 产品价格: | ¥1242.0 |
|---|---|---|---|
| 规格: | 10 mg | 产品价格: | ¥1800.0 |
| 规格: | 25 mg | 产品价格: | ¥2700.0 |
| 规格: | 50 mg | 产品价格: | ¥3780.0 |
| 规格: | 100 mg | 产品价格: | ¥5292.0 |
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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
, gv3101 - 25GEN 2. Transfer O/N culture to 200ml YEP in a sterile 500ml flask and shake at 250rpm until the OD is 0.3 (4-5hrs) 3. Spin in sterile 50ml screw cap tubes 4C 5krpm 10‚. Check to make sure cells are pelleted, if not repeat at higher
BACs, PACs, P1s and Plasmid Isolation Protocol (standard protocol)
实验试剂 Regents supplied by user 1. Sterile Deionized Water (or TE Buffer) 2. 70% Ethanol 3. 96%-100% Isopropanol 实验设备 Equipments supplied by user 1. Microcentrifuge capable
Protocol for competitive RT-PCR
, factor 2-2.5 between the standard amounts gives more accurate results (i.e.: 25 pg; 50 pg; 100 pg; 250 pg). In the following PCR 3-5 µl of cDNA, 1.5 units Taq DNA polymerase (Pharmacia), 200 µM of each dNTP, 250 nM of each primer and 1/10
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