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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
- 库存:
货期:询盘
- 供应商:
MedChemExpress LLC
- CAS号:
105102-22-5
- 规格:
询盘
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文献和实验配制(请具体参见淋巴细胞分离液使用说明):Solution AAnhydrous D-glucose 0.1 percent 1.0 g/lCaCl2 × 2H2O 5.0 × 10-5 M 0.0074 g/lMgCl2 × 6H2O 9.8 × 10-4 M 0.1992 g/lKCl5.4 × 10-3 M 0.4026 g/lTRIS 0.145 M 17.565 g/lDissolve in approximately 950 ml distilled water and add10
Construction and Characterization of Adenovirus Vectors
preset to 22°C, 37°C, 42°C, and 56°C METHOD Generation of Infectious Ad Plasmids 1. Generate a shuttle vector containing the transgene of interest using standard cloning procedures. 2. Digest 2 µg of the shuttle
CGH of Biotin Labeled DNA vs Digoxigenin Labeled DNA
to use probes which are a smear between 0.3 - 2.3 kb. Size can be adjusted by reducing or increasing the standard time for nick translation from 60 mins (using 2.5-5 ul of DNAse/Pol-1 enzyme mix. Paraffin samples also should be this size
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