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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
BSA PE Polypropylene Particles, 25 um
- 库存:
100
- 供应商:
百欧泰(biotyscience)
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文献和实验Western Blotting with Horseradish Pe
) is now ready for loading onto your gel. For Protein Concentration Determination of Whole Tissue 1.Rapidly homogenize every 0.25g tissue in 3.5ml of boiling lysis buffer (1% SDS, 1.0mM sodium ortho-vanadate, 10mM Tris pH 7.4). 2.Microwave for 10–15 seconds
T-cell dependent antigen specific in situ staining (Pe)
and then apply 3% human serum as a blocking agent (health hazard!). 4- incubate with the blocking for 10 min. 5- blot the slides without washing and apply Phycoerythrin (B-Pe or R-Pe) 0.5µg/ml in PBS-BSA-NaN 3 , in a moist chamber, at RT for 1-18 hr.
PriCells: 藻红蛋白标记抗体的方法﹠PE-标记蛋白A方法
G-50,收集蛋白A峰;3. 将0.77mg/ml的PE和0.27mg/ml蛋白A等量混合,于22℃下反应6h后,将混合物于4℃保存备用;4. 将以上两种PE标记制品溶于0.01mol/L pH7.4 PB(含有0.1mol/L DETA、1mol/L 碘乙酰胺、1%BSA和0.1% NaN3 )中,于0—4℃保存;
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