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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Protein A PMMA Particles
- 库存:
100
- 供应商:
百欧泰(biotyscience)
- 规格:
10 mg/ml10ml
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文献和实验Protein Assay (Spectrophotometer)
volume up to 800µl with H2O. Add 200µl dye (Biorad Protein Assay Dye Reagent Concentrate) Cover cuvettes with parafilm and mix gently. The colour of sample (intensity of blue) should be between the two extremes of the standard curve. Avoid
Biorad Protein Assay: Bradford
Biorad Protein Assay: Bradford Standards: 1 mg/ml BSA stock - dilute 1:10 to get 0.1 mg/ml BSA Add To get H-2O 20 µl 2 µg/ml 780 µl 40 µl 4 µg/ml 760 µl 60 µl 6 µg/ml 740 µl 80 µl 8 µg/ml 720 µl 100 µl 10
Bradford Protein Concentration Assay
of each BSA standard as a function of its theoretical concentration. The plot should be linear. Determine the best fit of the data to a straight line in the form of the equation "y = mx + b" where y = absorbance at 595 nm and x = protein concentration
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