- ￥2500
- 百欧泰(biotyscience)
- 北京
- ABT-31-0060-Protein A
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Protein A PMMA Particles
- 库存：
100
- 供应商：
百欧泰(biotyscience)
- 规格：
10 mg/ml10ml
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文献和实验protein. Grow 5ml of overnight culture at 37°C using LB broth (with 100mg/liter Amicillin). Next day add overnight culture to fresh LB (amp in it) at 1:20 ratio and till OD reaches 0.6-1.0 at fixed wavelength A 600. Induce expression of fusion
Protein Expression and Purification Protocol
in a shaker (250 RPM) at 37o C until the OD @ 600 nm is 0.5 to 1.5 OD. (Anywhere in this range is fine, however, extremely dense starter cultures>1.5 OD can cause problems in protein expression. Under these dense growth conditions the plasmid
Bacterial Protein Extraction (mini-scale)
Bacterial Protein Extraction (mini-scale)Using B-Per B-Per (Pierce,Cat number: 78248)Extraction of 1.5ml bact.culture OD600nm 1.5-3.5 A useful procedure for initial screening of expression conditions instead of sonication of bacteria
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