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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Protein A PMMA Particles
- 库存:
100
- 供应商:
百欧泰(biotyscience)
- 规格:
10 mg/ml10ml
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文献和实验Protein Syntheses in Cell Free Systems
per mg per ml at 260 nm. The ribosomes may be frozen and stored in liquid nitrogen, or used for in vitro protein synthesis. If frozen, they should be thawed only once prior to use. To test for protein synthesis, prepare
protein. Grow 5ml of overnight culture at 37°C using LB broth (with 100mg/liter Amicillin). Next day add overnight culture to fresh LB (amp in it) at 1:20 ratio and till OD reaches 0.6-1.0 at fixed wavelength A 600. Induce expression of fusion
Protein Expression and Purification Protocol
in a shaker (250 RPM) at 37o C until the OD @ 600 nm is 0.5 to 1.5 OD. (Anywhere in this range is fine, however, extremely dense starter cultures>1.5 OD can cause problems in protein expression. Under these dense growth conditions the plasmid
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