- ￥500 - 1250
- Solarbio
- K010183M
- 北京
- 2025年07月14日
- FCM
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
北京索莱宝科技有限公司
- 库存：
999
- 靶点：
CD3
- 目录编号：
K010183M
- 克隆性：
单克隆
- 抗体英文名：
Anti-CD3(PE) Monoclonal Antibody
- 抗体名：
CD3 epsilon; CD3e; CD3e antigen epsilon polypeptide (TiT3 complex); CD3E antigen epsilon polypeptide; CD3E antigen, epsilon subunit; CD3e molecule epsilon; CD3e molecule, epsilon (CD3 TCR complex); CD3e molecule, epsilon (CD3-TCR complex); CD3E_PIG; IMD18; T cell antigen receptor complex epsilon subunit of T3; T cell surface antigen T3/Leu 4 epsilon chain; T cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE
- 宿主：
Mouse
- 适应物种：
Human
- 亚型：
IgG2a, kappa
- 应用范围：
FCM
- 保存条件：
Store at 2-8℃. Avoid exposure to light.
- 浓度：
FCM 1X10^6cells+5 ul
- 规格：
25T/500T
| 规格： | 25T | 产品价格： | ￥500.0 |
|---|---|---|---|
| 规格： | 500T | 产品价格： | ￥1250.0 |
CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
Polyclonal and Monoclonal Antibodies
later again (2 + 2 week cycle). Monoclonal Antibody If titre is positive do one of the following 2 weeks later: I - Boost tail vein with 20 ug- 50 ug of Antigen in PBS and proceed with fusion on day 4. OR II - Boost
4. Remove solution and rinse ovaries with PBS 3 times, then wash 3 times for 10 minutes each 5. It may help antibody penetration to dissect apart the egg chambers 6. Incubate ovaries in PBT for 10 minutes 7. Add monoclonal supernatant
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