绿色/红色比率细胞强制降解试验-Green/Red Rati

绿色/红色比率细胞强制降解试验，Green/Red Ratiometric Cell Stress Assay，绿色/红色比率细胞强制降解试验,Green/Red Ratiometric Cell Stress Assay

产品名称：绿色细胞强制降解试验-Green/Red Ratiometric Cell Stress Assay

产品货号：MTA-U0901G-010

产品规格：10mL

检测化学或遗传压力，并确定化合物，扭转这种活细胞荧光检测应激反应。通过UPR的XBP 1/IRE 1臂测量ER应激反应。将细胞应力传感器添加到细胞中，并在第二天在荧光成像系统或自动酶标仪上测量稳健的信号。增加的绿色荧光指示ER应激或UPR活化。组成型表达的红色荧光蛋白可用于鉴定转导的细胞。该检测试剂在BacMam中进行遗传编码和包装，可在大多数细胞类型中进行有效转导-包括在HEK 293细胞中的均匀和接近100%的效率以及在许多原代或iPSC衍生细胞中的表达。该试剂盒包括ER应激传感器BacMam、500 mM丁酸钠水溶液和阳性对照激动剂（1 mM毒胡萝卜素DMSO溶液）。30 mL和60 mL的尺寸也可用。其他Montana分子工具，包括与神经变性相关的BacMam包装蛋白，如视紫红质，TDP-43和SOD 1，可以很容易地与该测定共表达。诸如cADDis cAMP测定的测定可用于研究应激细胞中信号传导的变化。

Detect chemical or genetic stress, and identify compounds that reverse the stress response with this live cell fluorescent assay. Measure ER stress responses via the XBP1/IRE1 arm of the UPR. Add the cell stress sensor to your cells and measure robust signals the next day on a fluorescence imaging system or automated plate reader. Increasing green fluorescence indicates ER Stress or UPR activation. Constitutively expressed red fluorescent protein can be used to identify transduced cells. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the ER stress sensor BacMam, 500mM sodium butyrate in H2O, and a positive control agonist (1mM thapsigargin in DMSO). 30mL and 60mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged proteins associated with neurodegeneration like Rhodopsin, TDP-43, and SOD1 can easily be co-expressed with this assay. Assays such as the cADDis cAMP assay can be used to investigate changes in signaling in stressed cells.

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